The primary method for quantitation and characterization of synthetic oligonucleotides is ion-pair reversed phase liquid chromatography (IP-RPLC). Mobile phase composition is typically an
alkylamine such as triethylamine (TEA) and perfluorinated alcohol such as hexafluoroisopropanol (HFIP), with methanol as the strong solvent. In this presentation, we will demonstrate the impact of
mobile phase optimization on chromatographic separation and MS sensitivity and spectral quality. Additionally, we will look into how temperature and flow-rate can impact oligo separations.

Subject area:
Biologics

Objectives (If applicable):
N/A

Target Audience (If applicable):
Analytical chemists
QA/QC analysts
R&D scientists, managers
Team members in CMC development projects
Manufacturing scientists, managers
Regulatory affairs specialists
Contract research organizations
Contract manufacturing organizations